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Scheme of cell exposure to endocytic inhibitors and surface-modified MNPs. At 75% to 80% confluence, cells were pre-treated (time 0) with endocytic inhibitor for 1 h in medium with 2% FBS, then (time 1) fresh medium with PEG-SO-MNPs or BSA-SO-MNPs in the presence of the same inhibitor was added for another period of 1 h. Cell exposure was finished by sucking off the culture medium (time 2) and washing the cells twice with phosphate-buffered saline (PBS).
