Table 2.
Enzymes of ganglioside biosynthesis and Glycologue reaction patterns.
| enzyme no. | EC number | short name | accepted namea | reaction patternb |
| 1 | EC 2.4.1.80 | UGCG | ceramide glucosyltransferase | UDP-G + T = UDP + GbT |
| 2 | EC 2.4.1.47 | β1Gal-T3 | N-acylsphingosine galactosyltransferase | UDP-L + T = UDP + LbT |
| 3 | EC 2.4.1.274 | β4Gal-T6 | glucosylceramide β-1,4-galactosyltransferase | UDP-L + GbT = UDP + Lb4GbT |
| 4 | EC 2.4.99.9 | ST3Gal-V | lactosylceramide α-2,3-sialyltransferase | CMP-S + Lb4GbT = CMP + [Sa3]Lb4GbT CMP-S + LbT = CMP + [Sa3]LbT |
| 5 | EC 2.4.99.8 | ST8Sia-I | α-N-acetylneuraminate α-2,8-sialyltransferase | CMP-S + [Sa3]Lb4*T = CMP + [Sa8Sa3]Lb4*T |
| 6 | EC 2.4.99.- [16] | ST8Sia-V | (α-2,8-N-acetylneuraminate α-2,8-sialyltransferase) | CMP-S + [Sa8Sa3]*T = CMP + [Sa8Sa8Sa3]*T CMP-S + Sa8Sa3*T = CMP + Sa8Sa8Sa3*T |
| 7 | EC 2.4.1.92 | β4GalNAc-T1 | (N-acetylneuraminyl)-galactosylglucosylceramide N-acetylgalactosaminyltransferase | UDP-V + Lb4*T = UDP + Vb4Lb4*T UDP-V + [Sa3]Lb4*T = UDP + Vb4[Sa3]Lb4*T |
| 8 | EC 2.4.1.68 | β3Gal-T4 | ganglioside galactosyltransferase | UDP-L + Vb4*T = UDP + Lb3Vb4*T |
| 9 | EC 2.4.99.- [17–18] | ST3Gal-II | (β-1,3-galactosyl-ceramide α-2,3-sialyltransferase) | CMP-S + Lb3Vb4*T = CMP + Sa3Lb3Vb4*T |
| 10 | EC 2.4.99.- [19] | ST6GalNAc-V | (α1,3-Sia-β1,3-Gal-β1,3-GalNAc α-2,6-sialyltransferase) | CMP-S + Sa3Lb3Vb4*T = CMP + Sa3Lb3[Sa6]Vb4*T |
aFor enzymes without an EC number, a suggested name is given in parentheses. Literature references supporting the unclassified activities are provided in the EC number column, after the EC sub-subclass. bAsterisks act as a wildcard character, to denote an unspecified portion of the oligosaccharide. Symbols and abbreviations used in reaction patterns are those of Table 1 with the following additions: UDP, uridine 5′-diphosphate; CMP, cytidine 5′-phosphate; CMP-S, CMP-N-acetyl-β-neuraminate; UDP-G, UDP-α-ᴅ-glucose; UDP-L, UDP-α-ᴅ-galactose; UDP-V, UDP-N-acetyl-α-ᴅ-galactosamine.