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. 2021 Mar 5;11(5):e3939. doi: 10.21769/BioProtoc.3939

Figure 1. Generation and characterization of human iPSC-derived NPCs.

Figure 1.

A. Representative images of neurospheres, neuroepithelial cells, and neural progenitor cells (NPCs). B. Image of a matrigel coated well. Scale bar: 200 µm. C. Immunocytochemistry of NPCs obtained from the control iPSC line XM001 ( Wang et al., 2018 ) stained for the neural progenitor markers NESTIN, DACH1, PAX6, and SOX2. Spontaneous differentiation of cells expressing TUJ1 or MAP2 can be present. Scale bar: 100µm. D. Quantitative real-time reverse transcription PCR (qRT-PCR) of neural progenitor markers SOX1, NESTIN, PAX6, and SOX2, and of pluripotency-associated markers OCT4, NANOG, DNMT3B, and DPPA4. Transcription levels in NPCs were normalized using GAPDH housekeeper gene (mean=SD +/-; n=3 technical replicates) and reported in relation to the transcription level of the respective control iPSC line XM001. E. Representative images of neural progenitor cells (NPCs) from passage 3 (p3) and passage 7 (p7) demonstrating the change in morphology towards homogenous NPC cultures. Scale bar: 100 µm.