Table 1. Basic media formulation.

Medium	Components	Volume	Stock conc.	Final conc.
Basic Medium 1 (BM1)	KnockOut-DMEM	40 ml	1×	1×
	KnockOut-SR	10 ml	5×	1×
	Pen/Strep	500 µl	10 mg/ml; 10.000 U/ml	0.1 mg/ml; 100 U/ml
	Glutamine	500 µl	200 mM	2 mM
	NEAA	500 µl	100×	1×
	Pyruvate	500 µl	100 mM	1 mM
	MycoZap	100 µl	500×	1×
Basic Medium sm- (base for sm+)	DMEM/F12	240 ml	1×	0.5×
	Neurobasal	240 ml	1×	0.5×
	N-2 Supplement	2.5 ml	100×	0.5×
	B-27 Supplement without vitamin A	5 ml	50×	0.5×
	Pen/Strep	5 ml	10 mg/ml; 10.000 U/ml	0.1 mg/ml; 100 U/ml
	Glutamine	5 ml	200 mM	2 mM
	MycoZap	1 ml	500×	1×