Table 2. Culture media formulation.

Medium	Components	Volume	Stock conc.	Final conc.
Medium 1 (M1); day 0-day 2	BM1	10 ml	1×	1×
	CHIR	5 µl	6 mM	3 µM
	SB	10 µl	10 mM	10 µM
	Dorsomorphin	2 µl	5 mM	1 µM
	Purmorphamine	7.69 µl	0.65 mM	500 nM
Medium 2 (M2); day 2-day 4	sm-	10 ml	1×	1×
	CHIR	5 µl	6 mM	3 µM
	SB	10 µl	10 mM	10 µM
	Dorsomorphin	2 µl	5 mM	1 µM
	Purmorphamine	7.69 µl	0.65mM	500 nM
M3/sm+ (maintenance of NPCs)	sm-	10 ml	1×	1×
	CHIR	5 µl	6 mM	3 µM
	Purmorphamine	7.69 µl	0.65 mM	500 nM
	Vitamin C	7.5 µl	200 mM	150 µM