FIGURE 5.

AS II restored the mitochondrial morphology and dynamics-associated proteins expression in diabetic rats. (A) Representative TEM micrographs of mitochondria morphology alterations in podocytes of each group (original magnification ×10,000, bars = 500 nm). (B–E) Representative photomicrographs and semiquantitative analysis of immunohistochemistry staining of Mfn2 and Fis1 (original magnification ×400). (F–K) Representative western blot and RT-qPCR analyses of Mfn2 and Fis1 in different groups (fold change); β-actin served as a loading control. Black arrowheads indicated changes in mitochondrial morphology. All data were expressed as mean ± SD. ∗p < 0.05 vs. control group; #p < 0.05 vs. STZ group.