Figure 7.

Characterization of splenic and lymphonodular T cell subpopulations during 24 weeks of feeding

(A–E) The frequencies of splenic (left column of scatter dot plots) and lymphonodular (right column of scatter dot plots) CD3⁺CD8⁻IFN-γ⁺ Th1 cells (A), CD3⁺CD8⁻IL-4⁺ Th2 cells (B), CD3⁺CD8⁻IL-17⁺ Th17 cells (C), CD4⁺CD25⁺FOXP3⁺ Tregs (D), and CD4⁺CD19⁻FOXP3⁺ICOS⁺CXCR5⁺ Tfr cells (E) (N ≥ 7 mice/group). All data are expressed as mean ± SEM and analyzed using unpaired two-tailed t test. ∗p < 0.05, ∗∗p < 0.01. See also Figure S4.