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Immunofluorescence staining and confocal microscopy of 4-HNE modified proteins presence and localization in breast tumor tissue and tumor-associated adipose tissue of normal-weight (black bars) and overweight/obese (gray bars) women with benign and malignant breast tumors. 4-HNE- (red) and nuclei Sytox Orange staining (false blue). Merged images are an overlay of two channels on phase-contrast tissue images. NC-negative control. Scale bars: 25 μm and 10 μm on merged x6 zoom.
