REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Fab	Dejnirattisai et al., 2021	N/A
IgG	Dejnirattisai et al., 2021	N/A
Human anti-NP (mAb 206)	Dejnirattisai et al., 2021	N/A
Regeneron mAbs	AstraZeneca	Cat#REGN10933, and REGN10987
AstraZeneca mAbs	AstraZeneca	Cat#AZD1061, AZD8895
Vir mAbs	Adagio	Cat#S309
Lilly mAbs	Adagio	Cat#Ly-CoV555, and Cat#Ly-CoV16
Adagio mAbs	Adagio	Cat#ADG10, Cat#ADG20, and Cat#ADG30
Anti-Human IgG (Fc specific)-Peroxidase	Sigma	Cat#A0170; RRID: AB_257868
Bacterial and virus strains
SARS-CoV-2 (Australia/VIC01/2020)	Caly et al., 2020	N/A
SARS-CoV-2/B.1.1.7	Public Health England	N/A
SARS-CoV-2/B.1.351	Public Health England	N/A
SARS-CoV-2/P.1	This paper	N/A
DH5α bacteria	In Vitrogen	Cat#18263012
Biological samples
Serum from Pfizer-vaccinated individuals	University of Oxford	N/A
Serum from AstraZeneca-Oxford-vaccinated individuals	University of Oxford	N/A
Plasma from SARS-CoV-2 patients	John Radcliffe Hospital in Oxford UK	N/A
Chemicals, peptides, and recombinant proteins
His-tagged SARS-CoV-2 RBD	This paper	N/A
His-tagged SARS-CoV-2 RBD K417N, E484K, N501Y	This paper	N/A
His-tagged human ACE2	This paper	N/A
Human ACE2-hIgG1Fc	This paper	N/A
Phosphate buffered saline tablets	Sigma-Aldrich	Cat#P4417
Dulbecco’s Modified Eagle Medium, high glucose	Sigma-Aldrich	Cat#D5796
Dulbecco’s Modified Eagle Medium, low glucose	Sigma-Aldrich	Cat#D6046
FreeStyle 293 Expression Medium	GIBCO	Cat#12338018
L-Glutamine–Penicillin–Streptomycin solution	Sigma-Aldrich	Cat#G1146
GlutaMAX	GIBCO	Cat#35050061
UltraDOMA PF Protein-free Medium	Lonza	Cat#12-727F
Fetal Bovine Serum	GIBCO	Cat#12676029
Polyethylenimine, branched	Sigma-Aldrich	Cat#408727
Carboxymethyl cellulose	Sigma	Cat#C4888
Strep-TactinXT	IBA Lifesciences	Cat#2-1206-025
HEPES	Melford	Cat#34587-39108
Sodium Chloride	Honeywell	Cat#SZBF3340H
LB broth	Fisher Scientific UK	Cat#51577-51656
Mem Neaa (100X)	GIBCO	Cat#2203945
Trypsin-EDTA	GIBCO	Cat#2259288
L-Glutamine 200 mM (100X)	GIBCO	Cat#2036885
SYPROorange (5000X in DMSO)	Thermo	Cat#S6651
Isopropyl β-d-1-thiogalactopyranoside	Meridian Bioscience	Cat#BIO-37036
Kanamycin	Melford	Cat#K22000
Lysozyme	Sigma-Aldrich	Cat#L6876
Tris-base	Melford	Cat#T60040
Imidazole	Sigma-Aldrich	Cat#56750
Triton X-100	Sigma-Aldrich	Cat#8787
Turbonuclease	Sigma-Aldrich	Cat#T4330
RNase A	QIAGEN	Cat#158922
NaCl	Sigma-Aldrich	Cat#S9888
MgSO4	Sigma-Aldrich	Cat#746452
Na2HPO4	Melford	Cat#S23100
NaH2PO4	Melford	Cat#S23185
Deposited data
The coordinates and structure factors of the SARS-CoV-2 wild type, K417N, K417T, P.1.1.7, P.1.351 and P.1 RBD with 222 and EY6A Fabs, and SARS-CoV-2 P.1 RBD with ACE2 crystallographic complexes	This paper	PDB:7NX6, 7NX7, 7NX8, 7NX9, 7NXA, 7NXB, 7NXC
Experimental models: cell lines
HEK293S GnTI- cells	ATCC	Cat#CRL-3022; RRID: CVCL_A785
HEK293 cells	ATCC	Cat#CRL-3216; RRID: CVCL_0063
Expi293F Cells	GIBCO,	Cat#A14527
HEK293T cells	ATCC	Cat#CRL-11268
Hamster: ExpiCHO cells	Thermo Fisher	Cat#A29133
Vero cells	ATCC	Cat#CCL-81; RRID: CVCL_0059
Recombinant DNA
Vector: pHLsec	Aricescu et al., 2006	N/A
Vector: pNEO	Aricescu et al., 2006	N/A
Vector: pOPING-ET	Nettleship et al., 2008	N/A
human ACE2 cDNA	Sourcebiosciences	Cat#5297380
Vector: human IgG1 heavy chain	German Cancer Research Center, Heidelberg, Germany (H. Wardemann	N/A
Vector: human lambda light chain	German Cancer Research Center, Heidelberg, Germany (H. Wardemann	N/A
Vector: human kappa light chain	German Cancer Research Center, Heidelberg, Germany (H. Wardemann	N/A
Vector: Human Fab	Univeristy of Oxford	N/A
Vector: Human scFv	University of Oxford, NDM (G. Screaton)	N/A
Software and algorithms
COOT	Emsley and Cowtan, 2004	https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
Xia2-dials	Winter et al., 2018	https://xia2.github.io/parameters.html
PHENIX	Liebschner et al., 2019	https://www.phenix-online.org/
PyMOL	DeLano	https://pymol.org/2/; RRID: SCR_000305
Data Acquisition Software 11.1.0.11	Fortebio	https://www.sartorius.com/en/products/protein-analysis/octet-systems-software
Data Analysis Software HT 11.1.0.25	Fortebio	https://www.sartorius.com/en/products/protein-analysis/octet-systems-software
Prism 8.0	GraphPad	https://www.graphpad.com/scientific-software/prism/; RRID: SCR_002798
IBM SPSS Software 26	IBM	https://www.ibm.com/us-en/?ar=1; RRID: SCR_019096
mabscape	This paper	https://github.com/helenginn/mabscape https://snapcraft.io/mabscape
Other
X-ray data were collected at beamline I03, Diamond Light Source, under proposal lb27009 for COVID-19 rapid access	This paper	https://www.diamond.ac.uk/covid-19/for-scientists/rapid-access.html
TALON Superflow Metal Affinity Resin	Clontech	Cat#635668
HiLoad 16/600 Superdex 200 pg	Cytiva	Cat#28-9893-35
Superdex 200 increase 10/300 GL column	Cytiva	Cat#28990944
HisTrap HP 5-ml column	Cytiva	Cat#17524802
HiTrap Heparin HT 5-ml column	Cytiva	Cat#17040703
Amine Reactive Second-Generation (AR2G) Biosensors	Fortebio	Cat#18-5092
Octet RED96e	Fortebio	https://www.sartorius.com/en/products/protein-analysis/octet-label-free-detection-systems
Buffer exchange system “QuixStand”	GE Healthcare	Cat#56-4107-78
Cartesian dispensing system	Genomic solutions	Cat#MIC4000
Hydra-96	Robbins Scientific	Cat#Hydra-96
96-well crystallization plate	Greiner bio-one	Cat#E20113NN
Crystallization Imaging System	Formulatrix	Cat#RI-1000
Sonics vibra-cell vcx500 sonicator	VWR	Cat#432-0137