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. 2021 Mar 29;218(6):e20201839. doi: 10.1084/jem.20201839

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© 2021 Davis et al.

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Figure 3. — Human aortic single-cell transcription profiling reveals elevated JMJD3 and inflammatory pathway expression in infiltrating monocytes/macrophages. (A) Cluster analysis using the uniform manifold approximation and projection technique of single-cell sequencing from human AAA (n = 4) and nonaneurysmal (n = 2) samples revealed 21 distinct cell clusters (representative). Source data are provided as a source data file. (B and C) Gene Ontology biological pathway enrichment analysis of differentially expressed genes up-regulated (B) and down-regulated (C) in AAA samples. The combined score metric corresponds to the P value (two-tailed Fisher’s exact test) multiplied by the Z-score of the deviation from the expected rank, and q values determined by Benjamini–Hochberg correction. (D) Feature plots displaying the single-cell gene expression of JMJD3 across cell clusters. (E) Fold expression analysis of JMJD3⁺ vs. JMJD3⁻ macrophages or monocytes (clusters 1–3) in AAA versus control tissue. (F) Focused Monocle pseudotime trajectory analysis including only the monocyte/macrophage defined clusters. Monocyte/macrophage clusters superimposed on pseudotime branches with gene expression plotted as a function of pseudotime. (G) Gene Ontology biological process, Reactome, or process enrichment analysis of differentially expressed genes JMJD3⁺ versus JMJD3⁻ cells. The combined score metric corresponds to the P value (two-tailed Fisher’s exact test) multiplied by the Z-score of the deviation from the expected rank, and q values were determined by Benjamini–Hochberg correction. (H) Heatmap of differentially expressed genes in JMJD3⁺ versus JMJD3⁻ macrophages from the cytokine-mediated signaling pathway.