Figure S2.

Inflammatory cytokines and murine elastase AAA model. (A) Quantitative PCR analysis of IL-23 from macrophages (CD11b⁺[CD3⁻CD19⁻Nk1.1⁻Ly6G⁻]) in mice infused with either saline or AngII for 28 d (n = 8–9 mice/group run in triplicate). *, P < 0.05 for Mann–Whitney U test. (B) ChIP analysis for H3K27me3 at Il23 promoter was performed (n = 5 mice/group run in triplicate). For all ChIP experiments, isotype-matched IgG was run in parallel. Dotted line represents isotype-matched control. *, P < 0.05 by ANOVA test with Newman–Keuls multiple comparison test. (C) Maximal infrarenal abdominal aortic diameter as determined by ultrasound in control and elastase-treated mice (n = 3–5 per group measured by two independent observers). *, P < 0.05; Mann–Whitney test. (D) Representative ultrasound images of the abdominal aorta at day 14 in control and elastase-treated mice. Dotted line represents aortic contour and arrows represent aortic wall diameter. Scale bar on the right side of image with each major axis representing 1 mm. (E) Quantitative PCR analysis of Il1b from macrophages (CD11b⁺[CD3⁻CD19⁻Nk1.1⁻Ly6G⁻]) in control or elastase mice (n = 3 mice/group). *, P < 0.05; Mann–Whitney test. (F) Fold change abdominal aortic diameter was determined by ex vivo measurement in Jmjd3^−/−MΦ or WTMΦ mice treated with or without elastase (n = 3 in control cohorts and n = 4 in elastase cohorts). *, P < 0.05; **, P < 0.001 by ANOVA with Newman–Keuls multiple comparison test.