Table 2.

Physicochemical characteristics of drug-loaded micelles.^a

Sample	DI water, pH 6.0					PBS, pH 7.4
	Deff (nm)	PDI	ζ-potential (mV)	LC (%, w/w) c		Deff (nm)	PDI
				SN38	DACHPt
SN38/m	45 ± 3	0.13 ± 0.03	−32 ± 5	12 ± 2	-	78 ± 6	0.18 ± 0.02
SN38 + DACHPt)/m (2:1) b	51 ± 3	0.12 ± 0.01	−17 ± 6	11	6	66 ± 5	0.15 ± 0.05
SN38 + DACHPt)/m (1:1) b	55 ± 5	0.15 ± 0.02	−7 ± 4	12 ± 2	12 ± 2	58 ± 2	0.16 ± 0.04

^aSize (D_eff), PDI, and ζ-potential were determined by DLS. All measurements were carried out at 1 mg/mL polymer concentration and 25°C. Data presented as mean ± SD, n=3.

^bDual-drug loaded micelles were prepared at different DACHPt loading. Drug content in the micelles expressed as (SN38 : DACHPt) weight ratios.

^cLoading capacity (LC) is expressed as mass of incorporated drug per mass of drug-loaded micelles (w/w). SN38 and DACHPt content were determined by UPLC and ICP-MS, respectively.