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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: J Immunol Methods. 2021 Jan 30;491:112970. doi: 10.1016/j.jim.2021.112970

Figure 2. Two-step ELISpot assay with in vivo expanded CD8 T cells.

Figure 2.

A two-step ELISpot assay approach was adapted to include a peptide pool matrix-screening step with a subsequent deconvoluted individual peptides analysis step for the screening of a large peptide library for potential T cell epitopes. Limited clinical sample was extended through an in vivo CD8 T cell expansion from PBMC; 7 day expanded CD8 T cells for the 1st ELISpot assays and an extra 3 days of expansion for 2nd ELISpot CD8 T cells.