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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: J Immunol Methods. 2021 Jan 30;491:112970. doi: 10.1016/j.jim.2021.112970

Figure 4. The comparison of 1st and 2nd ELISpot polyclonally expanded CD8 T-cell response specificities to HIV-1 gag peptide pools.

Figure 4.

Panels of 121 consensus and 320 global 15-mer peptides were organised into 15-pool and 18-pool 3D-matrices respectively. The first ELISpot screened large peptide pool panels using day 7 polyclonal expanded CD8 T cells and the positive peptide pools were re-confirmed on the second ELISpot assay using day 10 polyclonal expanded CD8 T cells. Positive pool responses between day 7 polyclonal expanded CD8 T cells and day 10 polyclonally expanded CD8 T cells from the same samples were correlated using spearman correlation rank test (top panels) and response magnitudes compared using wilcoxon matched pairs signed rank test (bottom panels): consensus (left panels) and global peptides (right panels). Each spot indicates the SFU magnitude of CD8 T cell response to a pool of HIV-1 gag peptides. The dashed (lower panels) line indicates the positive response threshold of 1.58 Log10 SFC/10E6 CD8 T cells.