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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: J Immunol Methods. 2021 Jan 30;491:112970. doi: 10.1016/j.jim.2021.112970

Figure 5. Cumulative distribution of ELISpot responses.

Figure 5

(A) The ELISpot assay limit of detection was defined as the lowest number of SFU that would distinguish a stimulated from unstimulated control well. The limited of detection was estimated using the means of triplicate medium control (mock) responses using polyclonal in vitro expanded CD8 T cell derived from HIV-1 positive participant samples PBMCs used at 2 × 105 cells per well. (B and C) Peptide pools were used to stimulate polyclonal in vitro expanded CD8 T cells from HIV-1 negative participants to assess the SFU distribution of negative responses that can be used to determine the cut-off for true positive sample responses observed with HIV-1 positive samples. Cumulative distribution of 40 HIV-1 negative sample SFU/106 CD8 T-cell ELISpot responses to both consensus (B) and global (C) peptide pools with inserts showing the medium, mean, SD and the 97.5 percentile of the HIV-1 negative samples’ responses.