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. 2021 Mar 29;28:22. doi: 10.1186/s12929-021-00720-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Expression of inflammatory mediators and reactive oxygen species-related enzymes in the aorta and PVAT. Expression of genes for chemokines, macrophage markers, and cytokines in the a aorta (n = 7, 5, and 6 for control, ob/ob, and ob/ob + Piog groups respectively) and b PVAT (n = 3, 5, and 6 for control, ob/ob, and ob/ob + Piog groups respectively). Expression of genes for ROS-scavenging enzymes and components of NADPH oxidase in the c aorta (n = 7, 5, and 6 for control, ob/ob, and ob/ob + Piog groups respectively) and d PVAT (n = 3, 5, and 6 for control, ob/ob, and ob/ob + Piog groups respectively). mRNA amount is expressed relative to the average expression in control mice. All data are resulted from 3-month-old male mice. ob/ob mice were treated with pioglitazone for 1 month starting at 2 months of age. *P < 0.05, **P < 0.01, and ***P < 0.001