Table 2.
Primer sequences for quantitative reverse transcription transcription-polymerase chain reaction
| Gene name | Forward | Reverse |
|---|---|---|
| Human NANOG | TGGGCCTGAAGAAAACTATCCAT | GAAGTGGGTTGTTTGCCTTTG |
| Human OCT4 | CCCGAAAGAGAAAGCGAACC | CCACACTCGGACCACATCC |
| Human SOX2 | ACCCCTGGCATGGCTCTT | ATGCTGATCATGTCCCGGA |
| Human P75 | CGACAACCTCATCCCTGTCTAT | CCACTGTCGCTGTGGAGTTTT |
| Human TFAP2B | CCGCCAAAGCCGTCTCT | TGGGTCGGCTGTTCCCT |
| Human TFAP2A | CCTGTCCAAGTCCAACAGCAAT | GACACTCGGGTGGTGAGAGC |
| Human SOX9 | CCCCAACAGATCGCCTACAG | TCTGGTGGTCGGTGTAGTCGT |
| Human SOX10 | CGCACCTGCACAACGCT | GCGGCCTTCCCGTTCTT |
| Human ZO-1 | AGGATCCATATCCCGAGGAAA | CGAGGTCTCTGCTGGCTTGT |
| Human Na+/K+ATPase | CAGGGCAGTGTTTCAGGCTAA | TCGACGATTTTGGCGTATCTT |
| Human SLC4A11 | GGACATCGCACGCAGGTT | CGTCATTGAGAGACCCGAAAG |
| Human AQP1 | CCTCCAGCTGGTGCTATGC | AAGGACCGAGCAGGGTTAATC |
| Human N-cadherin | ATCCGACGAATGGATGAAAGA | ACTAACAGGGAGTCATATGGTGGA |
| Human COL8A2 | CCGGCCACCTATACCTACGAT | TCCTGAAAAGGAGGAGTGGATGTA |
| Human P21 | GACAGCAGAGGAAGACCATGTG | GGCGTTTGGAGTGGTAGAAATC |
| Human P16 | TGCCCAACGCACCGAATA | GCACGGGTCGGGTGAGA |
| Human α-SMA | CAGAGACCCTGTTCCAGCCA | GCCCCCTGATAGGACATTGTTA |
| Human SNAIL2 | GCGATGCCCAGTCTAGAAAATCT | CTTCTCCCCCGTGTGAGTTCTA |
| Human GAPDGH | CATGTTCGTCATGGGTGTGAA | CATGGACTGTGGTCATGAGTCCT |