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. 2021 Mar 30;21:153. doi: 10.1186/s12887-021-02611-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — To evaluate intestinal transit at the population level, we categorized individuals within a population based on the location of tracer in the gastrointestinal tract. a Regions of the gut are divided to 4 zones based on anatomical landmarks. Zone 1 is the region of the intestinal bulb rostral to the swim bladder. Zone 2 is the region of the intestinal bulb ventral to the swim bladder. Zone 3 is the junction between the intestinal bulb and the mid intestine. Zone 4 consists of the mid- and posterior intestine. e, eye; sb, swim bladder. b Larvae were scored as zone 1, zone 2, zone 3, zone 4 and empty based on the anterior extent of tracer in the gastrointestinal tract (arrow). c Bar graphs representing the transit profile of a population of 7 dpf larvae at 3 h, 6 h, 9 h of transit time. Data are represented as the number of larvae classified into each zone category based on visual analysis of live larvae at the time specified. The difference of transit profiles of all time points between two groups show no statistically significant (P > 0.05)