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. 2021 Mar 29;18:83. doi: 10.1186/s12974-021-02134-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Representative images of F-actin labeling. WM-derived (a, b) and GM-derived microglia (c, d) left untreated or treated with LPS. Arrows indicate subtle differences in F-actin signals within the cells treated with LPS: open arrows highlight the stronger F-actin signal localized to the middle of the cell which was visible in nearly all GM-derived microglia treated with LPS and some WM-derived microglia treated with LPS; closed arrows point to a more diffuse and weaker staining of F-actin, visible in WM-derived microglia treated with LPS only. Confocal images with orthogonal slices showing the z-axis of a ramified microglia (e) and amoeboid microglia (f). Surface plots of the same ramified (g) and amoeboid (h) microglia showing the intensity of the rhodamine phalloidine signal (y-axis) which can be considered a measure for the amount of F-actin in the cell and the size of the cells in micrometers (x-axis). Scalebars (a–f) = 50 μm