(a) A dissected porcine heart showing the mitral valve (top) and tricuspid valve (bottom), with labels describing key anatomical components: valve leaflets, annulus, papillary muscles, and chordae tendineae (ruler shows inches). (b) Schematic of the excised leaflet and the central bulk region (top), and the mounted tissue specimen with preferred collagen fiber orientation on the biaxial mechanical testing system (C: circumferential direct, R: radial direction).