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Analysis and characterization of G5-SMTP polyplex formation. The ability of G5-SMTP to form polyplexes with pLuc was determined by (A) gel retention assay and (B) ethidium bromide exclusion assay. Characterization of (C) size and (D) surface charge were determined by dynamic light scattering (DLS) and measuring the zeta potential, respectively. Experiments were performed in triplicate, with data representing the means and standard error of the mean.
