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. Author manuscript; available in PMC: 2021 Mar 30.
Published in final edited form as: Mol Pharm. 2019 May 3;16(6):2376–2384. doi: 10.1021/acs.molpharmaceut.8b01313

Figure 3.

Figure 3.

In vitro cell contact assays. C2C12 cells were incubated for 15 min with pLuc polyplexes prepared using either G5 or G5-SMTP labeled with VivoTag 680XL. After incubation, cells were collected and analyzed for VivoTag 680XL fluorescence by flow cytometry. (A) Graphical representation of flow cytometry data obtained for charge ratios ranging from 5 to 20. Data represent the average of at least two experiments, with the error bars representing standard error. (B) Confocal studies of polyplex cell binding were performed by incubating C2C12 cells for 1 h with G5 or G5-SMTP polyplexes at a charge ratio of 10 that were labeled with VivoTag 680XL. After incubation, cells were washed and fixed with 4% paraformaldehyde prior to imaging with confocal microscopy. Bars:100 μ.