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. 2021 Mar 9;4(5):e202000997. doi: 10.26508/lsa.202000997

Figure S11. Collagen immunostaining and transcript levels in 2-mo WT and Hutchinson–Gilford Progeria Syndrome (HGPS) aortas.

Figure S11.

(A) Representative images of collagens-I, -III, -V immunostaining in 2-mo WT (n = 7) and Hutchinson–Gilford Progeria Syndrome (n = 7) aortic cross sections. Scale bar 100 mm. Medial layer is outlined in dashed lines and marked with “M.” (B) Quantification of immunostaining with results presented as fold change in fluorescent signal intensity for the medial and adventitial layers normalized to the mean intensity of the 2-mo WT media. Statistical significance between genotypes was evaluated by Mann–Whitney test. (C, D) Col1a1, Col3a1, and Col5a1 transcript levels in aortic (C) media layer (n = 4 per genotype) or (D) adventitia layer (n = 4 per genotype).