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. 2021 Mar 9;4(5):e202000997. doi: 10.26508/lsa.202000997

Figure S7. Collagen and lysl oxidase (LOX) abundance in PBS and BAPN-treated mice.

Figure S7.

(A) Representative Transmission Electron Microscopy images (50,000× magnification) of cross sections from 2-mo Hutchinson–Gilford Progeria Syndrome (HGPS) carotid arteries that had been treated with vehicle (PBS) or BAPN as described in the Materials and Methods section. Scale bar = 200 nm. (B) Quantification of collagen area within the elastin folds as described in Supplemental Data 1 (31KB, docx) and Fig S5 (n = 3–4 mice per treatment with 10 elastin folds analyzed per mouse). (C, D) Aorta lysates, prepared from 2-mo HGPS mice treated with PBS or BAPN, were analyzed by Western blotting and probed for LOX and FAK (loading control). (C) Representative Western blot; white spaces indicate removal of extraneous data. (D) Quantification of LOX signal intensities, normalized to the loading control, in HGPS mice treated with PBS (n = 4) or BAPN (n = 4).