Figure 3. Stress granules contain K48- and K63-linked Ub chains.

(A, B) HeLa cells were subjected to treatment with arsenite, VER/Puro and heat stress as indicated. (A, B) The colocalization between G3BP and K48-linked Ub chains (A) or K63-linked Ub chains (B) was visualized by confocal immunofluorescence microscopy. Representative Ub-positive stress granules are marked by arrows or magnified in the inset. Scale bar, 10 μm.