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. Author manuscript; available in PMC: 2021 Mar 30.
Published in final edited form as: J Vis Exp. 2020 May 7;(159):10.3791/60965. doi: 10.3791/60965

Figure 1: Representative example for calculating angled coordinates targeting the hypothalamic ventromedial nucleus.

Figure 1:

(angles and line segments not drawn to scale). A. This length should be calculated using basic trigonometry. Per this example, A = 2.03mm. B. Estimated length based upon assignment of arbitrary axis of rotation. Per this example, B = 7.576mm. C. Calculated hypotenuse. Note that the depth of fiberoptic/needle insertion used will depend upon desired proximity to target region and requires optimization. Modified from Kopf Carrier #96.