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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Leukemia. 2020 Jun 30;35(3):679–690. doi: 10.1038/s41375-020-0937-3

Figure 2. Prl3 collaborates with Myc to accelerate T-ALL onset and suppresses apoptosis in transgenic zebrafish.

Figure 2.

(A) Syngeneic CG1 fish injected at the one-cell stage with rag2-Myc or rag2-prl3 and rag2-Myc in combination. Representative images of mosaic transgenic zebrafish shown at 28 days post injection. (B) Kaplan-Meier analysis of leukemic fish (p=0.012 comparing Myc, prl3 and Myc+prl3 expressing T-ALL, log-rank statistic). n≥ 7 animals/arm. (C) Histological analysis of representative T-ALL. Hematoxylin and eosin stained sections juxtaposed to immunohistochemistry for phospho-H3 and cleaved caspase3. Yellow arrowheads denote examples of positively stained cells. (Right) Quantification of IHC data where each datum point represents a single primary T-ALL (filled) or transplanted leukemia (open). ** denotes p<0.01 by Student’s t-test. Not significant (NS). Scale bar equals 0.1cm.