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. Author manuscript; available in PMC: 2021 Jul 1.
Published in final edited form as: Nat Aging. 2021 Jan 14;1(1):73–86. doi: 10.1038/s43587-020-00006-2

Extended Data Fig. 5. A Low BCAA diet reduces mTORC1 activity in male, but not female, muscle.

Extended Data Fig. 5

(A-B) mTORC1 activity determined by Western blotting and quantification of muscle tissue lysates from male and female mice. Young (12 months females; 15 months males) and aged (22 months females; 25 months males) mice were fed either a Control or Low BCAA diets from 6.5 months of age for young and 16 months of age for aged mice, then sacrificed following an overnight fast followed by 4 hours of refeeding. (A) Male and (B) Female muscle. Quantification was by ImageJ (N = 3 biologically independent animals for all groups). (A-B) * p < 0.05 (p-values for (B); pS6/S6, Young CTL vs. Young LBC=0.0025; Young CTL vs. Aged CTL=0.0327; pS6K1/S6K1, Aged CTL vs. Aged LBC=0.0254). Statistics for the overall effects of diet, age and the interaction represent the p value from a two-way repeated measures ANOVA, multiple comparisons by two-sided Sidak’s post-test. Full scans of the cropped western blots shown here are provided as Source Data files. CTL=Control, LBC=Low BCAA. Data are represented as mean ± SEM.