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. 2021 Mar 28;13(1):1902719. doi: 10.1080/19490976.2021.1902719

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© 2021 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Ferroptosis is present in and enhances intestinal organoid H/R injury. (a-b) Total GSH and the ratio of total GSH/GSSG in the organoids. (c-d) MDA and Fe²⁺ levels in the organoids. (e) The protein levels of Gpx4, Fth1 and Cox-2 in the organoids, scale bar is 20 μm. (f-h) Relative fluorescence quantitative statistics of Gpx4, Fth1 and Cox-2 protein expression. (i) Ileum organoid morphology observed by light microscopy and HE staining and the expression of Occludin and ZO-1 in the organoids observed by immunofluorescence, scale bar is 20 μm. (j) Organoid viability measured by CCK-8. (k) Levels of lactate dehydrogenase (LDH) released into the medium. (l-m) Relative fluorescence quantitative statistics of Occludin and ZO-1 protein expression in the organoids. The results are expressed as the mean ± SEM. n = 6. * p < .05, ** p < .01, *** p < .001 by one-way ANOVA (Tukey’s test)