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. Author manuscript; available in PMC: 2021 May 23.
Published in final edited form as: Nat Methods. 2020 Nov 23;17(12):1222–1228. doi: 10.1038/s41592-020-00999-z

Extended Data Fig. 1. Theoretical Analysis of Peptide Complexity Reduction by Gas-phase Fractionation quadrupole isolation width and FAIMS compensation voltage (CV).

Extended Data Fig. 1

This analysis uses the maximum CV signal for all peptide precursor masses identified from stepped CV analysis of the human proteome. Stacked barplots show the number of peptide precursor masses per bin split by the contribution from each FAIMS CV fraction. The top panel shows precursor masses per 4 m/z isolation bin. The middle panel shows a roughly linear decrease in the maximum number of peptide precursor masses when the isolation width is decreased to 2 m/z. The bottom panel shows a nonlinear decrease in the number of peptide precursor masses due to selection by FAIMS gas phase fractionation at constant quadruople isolation width.