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. 2021 Mar 29;8(3):e987. doi: 10.1212/NXI.0000000000000987

Figure 4. Anti-RGS8 Autoantibody Detection in Patient Sera and CSF by Different Immunoassays.

Figure 4

(A) Immunoblots with lysates of RGS8-transfected HEK293 cells incubated with 1:200 diluted patient or control sera and anti-human IgG-AP or anti-His antibody and anti-mouse IgG-AP. (B) Results of RGS8 line blot assay with patient or control sera (CS) (1:100). (C) ELISA with purified recombinant RGS8-His with patient or control sera (1:100) and patient or control CSF (1:10) and anti-human IgG POD. Positive and total numbers of sera and CSF are given below the diagrams. (D) IFA with patient or control sera (1:100) and RGS8- or Mock-transfected HEK293 cells incubated with anti-human IgG-Alexa488. Nuclei were counterstained by incubation with TO-PRO-3 iodide (blue) (scale bar 50 µm).