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. Author manuscript; available in PMC: 2022 May 1.
Published in final edited form as: J Immunol Regen Med. 2021 Feb 2;12:100038. doi: 10.1016/j.regen.2021.100038

Figure 2: Exposure of macrophages to apoECs induces macrophage phenotype changes in vitro.

Figure 2:

Serum starvation-induced apoptotic GFP-hECs or hECs were incubated with PMA-induced THP-1 macrophages for 6 and 24 hours. A) CD80, CD163 and CD206 expression on apoptotic GFP-hECs treated THP-1 macrophages by flow cytometry analysis. Cells were pooled from three wells of a 6 well plate at each time point. The number shown in the upper right on each scatter plot is the percentage of the target on F4/80 positive macrophages. Gating strategies are shown in Appendix B. B). mRNA expression of TNF-α, IL-1β, IL-6, IL-10, iNOS, TGF- β1, VEGF-A, and IGF-1 in apoptotic hECs treated THP-1 cells. The level of relative expression for each target at zero hour without treatment was used as the baseline control. N=3 triplicate wells at each time point. Representative results from two experiments are shown. Two-way ANOVA followed by Bonferroni’s post hoc test was used for statistical analysis. *p<0.05, **p<0.01