WT or SK1−/− mice were fed alcohol
diet and sacrificed 15 days later.
(A) H&E staining and Oil red O staining of liver.
(B) Serum ALT and AST.
(C) Hepatic triglycerides.
(D) Frequencies of immature myeloid cells, macrophages,
Th1, and Th17 cells in the liver.
(E) Immunohistochemistry staining of myeloperoxidase (MPO)
in the liver.
(F) Real-time PCR analysis of the relative expression of
indicated genes in the liver.
Data in all panels are presented as Mean ± SEM. n=11 *
P<0.05; ** P<0.01