(A) H&E staining of small and large intestine.
(B) Alcian Blue staining and immunohistochemistry staining
of mucin 2 in colonic tissue.
(C) Plasma levels of Dextran-FITC for gut permeability.
(D) Real-time PCR analysis of the expression of indicated
genes in the colon (left) and IL-17A and Rorc mRNAs (right) in colonic LPLs.
(E) ELISA analysis of IL-17A or IL-23 in the supernatant of
colonic LPLs.
(F) Intracellular staining of IL-17A+ and
IFN-γ+ CD4+ T cells from gut LPL.
(G) Relative levels of S1P in the ileum of alcohol-fed
mice.
(H) Immunofluorescence was used to evaluate the expression
of pSTAT3 in the ileum.
Data in all panels are presented as Mean ± SEM. n=11 *
P<0.05; ** P<0.01