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. 2021 Mar 26;13:2835–2848. doi: 10.2147/CMAR.S294584

Figure 1.

Figure 1

Sensitivity and specificity of PCR assay for detection of MMTV DNA-like env sequences extracted from MCF-7 cell line. (A) Ethidium bromide–stained gel electrophoresis shows sensitivity assay of primers used to amplify MMTV DNA-like env sequences. Positive signals are 250 bp. Lane M = 100-bp ladder; lanes 1–5 = positive lanes of serial dilutions from 10−1-10−5. (B) Ethidium bromide–stained gel electrophoresis of specificity assay of primers used to amplify MMTV DNA-like env sequences. Positive signal at 250 bp. For MMTV DNA product (lane 2), lanes 1, 3, 4, 5 are negatives, Lane M = 100bp ladder.