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. 2021 Mar 26;13:2821–2834. doi: 10.2147/CMAR.S293322

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© 2021 Li et al.

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NOP14-AS1 downregulation restricts TSCC cell proliferation, migration, and invasion but promotes cell apoptosis via the miR-665/HMGB3 axis. (A and B) CCK-8 assay and flow cytometric analysis were used to analyse the proliferation and apoptosis of CAL-27 and SCC-15 cells after transfection with si-NOP14-AS1 together with miR-665 inhibitor or NC inhibitor. (C) Migration and invasion of CAL-27 and SCC-15 cells treated as above described were tested by Transwell migration and invasion assays. (D) HMGB3 protein expression in pcDNA3.1-HMGB3-transfected or pcDNA3.1-transfected CAL-27 and SCC-15 cells was determined by Western blotting. (E–G) Cotransfection of si-NOP14-AS1 and pcDNA3.1-HMGB3 or pcDNA3.1 was performed in CAL-27 and SCC-15 cells. The proliferation, apoptosis, migration, and invasion were evaluated by CCK-8 assay, flow cytometric analysis, and Transwell migration and invasion assays, respectively. *P < 0.05; **P < 0.01, compared with control.

Abbreviations: NOP14-AS1, NOP14 antisense RNA 1; NC inhibitor, negative control miRNA inhibitor; miR-665, microRNA-665; HMGB3, high mobility group box 3; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; si-NC, negative control small interfering RNA; si-NOP14-AS1, small interfering RNA targeting NOP14-AS1.