Fig 3. Flagellin modulate the production of IL-22 cytokines in splenocytes from NTHi-infected cigarette smoke-exposed mice.

(a) IFN-γ, IL-17 and IL-22 concentrations were measured in the supernatants of unstimulated spleen cells of mice infected or not with NTHi and treated or not with FliC, at 48h after infection. (b) IFN-γ, IL-17 and IL-22 concentrations were measured in the supernatants of activated spleen cells of mice infected or not with NTHi and treated or not with FliC, at 48h after infection. Spleen cells were activated by addition of heat-killed-NTHi (MOI 10) during 48 hours. Two independent experiments have been performed with at least 3–4 mice in each group. *: p<0.05 and **: p<0.01.