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. 2021 Mar 30;16(3):e0236216. doi: 10.1371/journal.pone.0236216

Table 2. List of the antibodies and of the ELISA kits used in this study.

Flow cytometry mAb Target Manufacturer Catalog Nb
FITC- I-Ab Miltenyi Biotech 130-102-168
PE-F4/80 Miltenyi Biotech 130-102-422
PerCP-Cy5.5—CD103 BD Biosciences 563637
PE-Cy7—CD11c BD Biosciences 558079
APC—CCR2 Miltenyi Biotech 130-119-658
AF700—CD86 BD Biosciences 560581
APC-H7- Ly6G BD Biosciences 560600
V450—CD11b BD Biosciences 560455
VioGreen—CD45 Miltenyi Biotech 130-110-665
BV605—Ly6C Biolegend 128036
BV786—CD64 BD Biosciences 741024
PE-CF594—SiglecF BD Biosciences 562757
FITC—CD5 Miltenyi Biotech 130-102-574
Tetramer mCD1d 167ms NIH facility 30663
PerCP-Cy5.5—NK1.1 Miltenyi Biotech 130-103-963
PE-Cy7—CD4 Miltenyi Biotech 130-102-411
APC—CD25 Miltenyi Biotech 130-102-550
AF700—CD69 BD Biosciences 561238
APC-Vio770—TCRγδ Miltenyi Biotech 130-104-016
VioBlue -TCRβ Miltenyi Biotech 130-104-815
V500—CD8 BD Biosciences 130-109-252
BV605—CD45 Biolegend 103140
ELISA kits Target Manufacturer Catalog Nb
IFN-γ ELISA kit Invitrogen 88-7314-88
IL-1β Duoset Biotechne DY401
IL-6 ELISA kit Invitrogen 88-7064-88
IL-17 ELISA kit Invitrogen 88-7371-88
IL-22 Duoset Biotechne DY582
IL-23 ELISA kit Invitrogen 88-7230-88
TNF-α ELISA kit Invitrogen 88-7371-88
Defensin-β2 Abbexa Abx254734