Table 2. List of the antibodies and of the ELISA kits used in this study.
Flow cytometry mAb | Target | Manufacturer | Catalog Nb |
---|---|---|---|
FITC- I-Ab | Miltenyi Biotech | 130-102-168 | |
PE-F4/80 | Miltenyi Biotech | 130-102-422 | |
PerCP-Cy5.5—CD103 | BD Biosciences | 563637 | |
PE-Cy7—CD11c | BD Biosciences | 558079 | |
APC—CCR2 | Miltenyi Biotech | 130-119-658 | |
AF700—CD86 | BD Biosciences | 560581 | |
APC-H7- Ly6G | BD Biosciences | 560600 | |
V450—CD11b | BD Biosciences | 560455 | |
VioGreen—CD45 | Miltenyi Biotech | 130-110-665 | |
BV605—Ly6C | Biolegend | 128036 | |
BV786—CD64 | BD Biosciences | 741024 | |
PE-CF594—SiglecF | BD Biosciences | 562757 | |
FITC—CD5 | Miltenyi Biotech | 130-102-574 | |
Tetramer mCD1d 167ms | NIH facility | 30663 | |
PerCP-Cy5.5—NK1.1 | Miltenyi Biotech | 130-103-963 | |
PE-Cy7—CD4 | Miltenyi Biotech | 130-102-411 | |
APC—CD25 | Miltenyi Biotech | 130-102-550 | |
AF700—CD69 | BD Biosciences | 561238 | |
APC-Vio770—TCRγδ | Miltenyi Biotech | 130-104-016 | |
VioBlue -TCRβ | Miltenyi Biotech | 130-104-815 | |
V500—CD8 | BD Biosciences | 130-109-252 | |
BV605—CD45 | Biolegend | 103140 | |
ELISA kits | Target | Manufacturer | Catalog Nb |
IFN-γ ELISA kit | Invitrogen | 88-7314-88 | |
IL-1β Duoset | Biotechne | DY401 | |
IL-6 ELISA kit | Invitrogen | 88-7064-88 | |
IL-17 ELISA kit | Invitrogen | 88-7371-88 | |
IL-22 Duoset | Biotechne | DY582 | |
IL-23 ELISA kit | Invitrogen | 88-7230-88 | |
TNF-α ELISA kit | Invitrogen | 88-7371-88 | |
Defensin-β2 | Abbexa | Abx254734 |