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. 2021 Mar 18;17(3):e1009438. doi: 10.1371/journal.ppat.1009438

Fig 3. circDtx1 regulates miR-15a-5p expression and activity.

Fig 3

(A) The Ago2-RIP assay for the amount of circDtx1 in MIC cells transfected Ago2-flag or pcDNA3.1-flag. (B) A schematic illustration showing overlapping of the target miRNAs of circDtx1 predicted by TargetScan, mIRanda, and RNAhybrid. (C) Schematic drawing showing the putative binding sites of the miRNAs associated with circDtx1. (D and E) Relative expression of candidate miRNAs in MIC and MKC cells transfected with oe-circ (D) and si-circ (E), respectively. (F) circDtx1 reduces miR-15a-5p activity. The relative luciferase activity was analyzed in MKC cells co-transfected with mimics, circDtx1 overexpression plasmid, and control vector, together with miR-15a-5p sensor. All data represented the mean ± SD from three independent triplicated experiments. **, p < 0.01.