Skip to main content
. 2021 Mar 18;17(3):e1009438. doi: 10.1371/journal.ppat.1009438

Fig 7. circDtx1 acts as a sponge of miR-15a-5p to enhance TRIF expression.

Fig 7

(A and B) Relative mRNA and protein levels of TRIF in MIC or MKC cells after cotransfected with NC, si-circ, vector, or oe-circ by western blot (A) and qPCR assays (B). (C) Relative mRNA levels of TRIF in MIC or MKC cells after cotransfected with si-circ-NC, si-circ, vector, or oe-circ by qPCR assay. (D) The relative luciferase activities were detected in EPC cells after cotransfection with TRIF 3’UTR luciferase reporter vector, NC, mimics, oe-circ or oe-circ-mut. (E) Western blot assays were detected in MKC cells after cotransfection with TRIF overexpression plasmid, NC, mimics, or oe-circ. (F) oe-circ counteracts the negative effect of miR-15a-5p. The relative luciferase activities were detected in MIC cells after cotransfection with TRIF expression plasmid, phRL-TK Renilla luciferase plasmid, luciferase reporters, NC, mimics, or oe-circ. (G) Cell proliferation was assessed by EdU assays in MIC cells after cotransfected with NC, mimics, or oe-circ. (H) Cell viability was assessed by ATP viability assays in MIC cells after cotransfected with NC, mimics, or oe-circ. (I) SCRV RNA expression was assessed by qPCR in MIC cells after cotransfected with NC, mimics, or oe-circ. All data represented the mean ± SD from three independent triplicated experiments. *, p < 0.05; **, p < 0.01.