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. 2021 Mar 18;17(3):e1009438. doi: 10.1371/journal.ppat.1009438

Fig 8. The ceRNA network of regulating TRIF is widely found in teleost fish.

Fig 8

(A) Sequence alignment of circDtx1 from various teleost fish species. (B) Sequence alignment of TRIF containing miR-15a-5p binding site among different teleost species. (C) miR-15a-5p regulating the luciferase activity of TRIF-3’UTR is examined in L. croceas and S. ocellatus. (D) The relative luciferase activities were detected in EPC cells after cotransfected with LcrcircDtx1-wt or LcrcircDtx1-mut and mimics or NC (left panel) and cells after cotransfected with SoccircDtx1-wt or SoccircDtx1-mut and mimics or NC (right panel). (E) LcrcircDtx1 and SoccircDtx1 reduces miR-15a-5p activity. EPC cells were transfected with LcrcircDtx1 or SoccircDtx1 expression plasmid, mimics, control vector, together with TRIF-3’UTR, the luciferase activity was analyzed and normalized to renilla luciferase activity. All data represented the mean ± SD from three independent triplicated experiments. *, p < 0.05.