Figure 5.

Data acquired using droplets of 60 μM ubiquitin and different acquisition modes on the SYNAPT at varying microdroplet infusion rates. Each mode is accompanied by a mass spectrum extracted from one droplet. (A) TIC obtained from a microdroplet infusion rate of 11 Hz acquired in standard MS mode with a scan time of 0.016 s (1s, ∼40 scans shown). (B) TIC obtained at the same infusion rate but acquired using the fast-scanning acquisition mode (1 s, 200 scans shown). (C) TIC obtained using the fast-scanning mode but at an increased infusion rate of 33 Hz (1 s, 200 scans shown).