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. Author manuscript; available in PMC: 2021 Sep 15.
Published in final edited form as: Acta Biomater. 2020 Jul 8;114:407–420. doi: 10.1016/j.actbio.2020.07.006

Fig. 4.

Fig. 4

MTT assay showing the effects of soy isoflavones loaded 3DP TCP bone tissue engineering scaffolds for in vitro osteosarcoma cell proliferation in a static condition at day 3, 7 and 11. Lowest osteosarcoma or bone cancer cell viability can be seen in the presence of only genistein, while daidzein and glycitein could not exert a significant chemopreventive effect on day 3 or day 7. Combination of all three isoflavones still showed significant cellular toxicity. [* denotes p-value <0.05, ** denotes p-value <0.0001, statistically significant difference between control and test sample]. [Control: 3D TCP: 3D printed porous TCP scaffold]