Fig. 5.

FESEM images showing the effects of soy isoflavones loaded 3DP TCP bone tissue engineering scaffolds for in vitro MG-63 cell proliferation in a static condition at day 3, 7 and 11. Layers of bone cancer cells can be seen on TCP scaffold alone, where the presence of genistein exhibits very low cell proliferation suggesting its chemopreventive ability. Although daidzein and glycitein did not have a pronounced effect on MG-63 cells on day 7, the combined effect of three isoflavones showed significant chemopreventive effects in all the time points.