Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Mar 26;14:1125–1143. doi: 10.2147/JIR.S304461

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 Feng et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

PMC Copyright notice

Effect of LPS on the expression of miR-138-5p and NLRP3/caspase-1 in RM microglial cells. The qRT-PCR result of (A) miR-138-5p, (B) NLRP3, caspase-1, IL-1β and IL-18 mRNA expression in RM cells at 6 h, 12 h and 24 h after LPS. (C) ELISA assay of IL-1β and IL-18 protein expression. (D) Representative Western blot bands and densitometric analysis of (E) NLRP3 and (F) caspase-1 in RM cells at 6 h, 12 h and 24 h after LPS. (G) Hoechst 33342/PI dual staining assays and counted of cell apoptosis in RM cells at 6 h, 12 h and 24 h after LPS. GAPDH was used as an internal control. Data were presented as mean ± SEM. n = 6 per group. *p < 0.05, **p < 0.01, ***p < 0.001.

Abbreviation: LPS, lipopolysaccharide; RM, rat microglia; PI, propidium iodide.