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. 2021 Mar 26;14:1125–1143. doi: 10.2147/JIR.S304461

Figure 5.

Figure 5

Effect miR-138-5p on LPS-induced inflammatory response in RM microglial cells. The qRT-PCR result of (A) miR-138-5p, (B) NLRP3, (C) caspase-1, (D) IL-1β, and (E) IL-18 mRNA expression in LPS-treated RM microglial cells after pretreatment with miR-138-5p inhibitor or mimic. (F–G) ELISA result of IL-1β and IL-18 protein expression after pretreatment with miR-138-5p inhibitor or mimic in LPS-treated RM microglial cells. (H–I) Hoechst 33342/PI dual staining assays and counted of cell apoptosis in LPS-treated RM microglial cells after pretreatment with miR-138-5p inhibitor or mimic. (J) Representative Western blot bands and densitometric analysis of (K) NLRP3 and (L) caspase-1 after pretreatment with miR-138-5p inhibitor or mimic in LPS-treated RM microglial cells. Data were presented as mean ± SEM. n = 6 per group. *p < 0.05, **p < 0.01, ***p < 0.001.

Abbreviations: LPS, lipopolysaccharide; RM, rat microglia; NC, negative control; PI, propidium iodide.