Table 1.
Specific Primers Sequences of mRNAs
| Gene | GenBank Accession |
Primer Sequences (5ʹ-3ʹ) | Product Size (bp) |
|---|---|---|---|
| NLRP3 | NM_001191642.1 | F: CCAGGAGTTCTTTGCGGCTA | 145 |
| R: GCCTTTTTCGAACTTGCCGT | |||
| Caspase-1 | NM_012762.3 | F: CTGGAGCTTCAGTCAGGTCC R: CTTGAGGGAACCACTCGGTC |
141 |
| IL-1β | NM_031512.2 | F: CTTGACTTGGGCTGTCCAGA | 133 |
| R: ACGGGCAAGACATAGGTAGC | |||
| IL-18 | NM_019165.2 | F: CAACCGCAGTAATACGGAGC | 106 |
| R: GATTCGTTGGCTGTTCGGTC | |||
| Iba1 | NM_017196.3 | F: CAACAAGCACTTCCTCGATGATC | 71 |
| R: TGAAGGCCTCCAGTTTGGACT | |||
| β-actin | NM_031144.3 | F: TGTCACCAACTGGGACGATA | 165 |
| R: GGGGTGTTGAAGGTCTCAAA |
Abbreviations: F, forward; R, reverse; bp, base pair.