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. Author manuscript; available in PMC: 2021 Nov 16.
Published in final edited form as: Curr Biol. 2020 Sep 24;30(22):4454–4466.e5. doi: 10.1016/j.cub.2020.08.099

Figure 2. Extended LP motif sequences impart variation in cyclin recognition.

Figure 2.

(A) LP motif sequences used throughout this study.

(B) Four blocks of 4 residues in the Ste5 LP motif region were mutated (to AAAA) and tested for effects on substrate phosphorylation (as in Figure 1B) induced by Cln2 or Ccn1 cyclins.

(C) Cln1/2 and Ccn1 cyclins were tested for phosphorylation of substrates with LP motifs from Ste5 or Sic1. Also see Figure S2.

(D) PGAL1-cyclin strains harboring Ste20Ste5PM chimeras with different LP motifs were tested for pheromone-induced MAPK phosphorylation. Below, quantified results (mean ± SEM, n = 4).