Video 3. Time-lapse images of traction force magnitude for a ChoK1 cell expressing GFP-talin.

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Traction forces are reconstructed from the bead images using high-resolution, L1-regularized, TFM software (Han et al., 2015). The color scale is the same as one at Figure 1a, that is, 0 (blue) – 300 Pa (red). The time interval per frame: 1.644 s. Playing speed: 25 frames/s. Duration of the movie: 8 min 13 s. Scale bar: 5 μm.