Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| E. coli | BL21(DE3) | Sigma-Aldrich | CMC0016 | Electrocompetent cells |
| Cell line (Cricetulus griseus) | CHO-K1 | ATCC | CCL-61 | Courtesy of Dr.Horwitz, Allen Institute |
| Cell line Mus musculus |
IMCD Talin1/2 KO | This paper | Courtesy of Dr. Zent, Vanderbilt University | |
| Lentiviral expression vector | pLVX-shRNA1 | Clontech | Lentiviral construct for stable expression of shRNA. | |
| Lentiviral expression vector | pLVX-CMV-100 | Addgene | Catalog # 110718 | Lentiviral construct for stable expression of ectopic proteins. |
| Transient expression vector | pCDNA3.1(+) | ThermoFisher Scientific | Catalog # V79020 | DNA construct for transient expression of ectopic proteins. |
| Primary antibody | Anti-talin 1 | Abcam | Catalog # 71333 | WB 1:1000 |
| Primary antibody | Anti-talin 2 | Abcam | Catalog # 105458 | WB 1:1000 |
| Secondary antibody | Goat anti-Mouse IgG (H+L) Cross-Adsorbed- Perosidaxe Antibody | ThermoFisher Scientific | Catalog # G-21040 | WB 1:1000 |
| Secondary antibody | Goat anti-Rabbig IgG (H+L) Cross-Adsorbed-Peroxidase Antibody | ThermoFisher Scientific | Catalog # G-21234 | WB 1:1000 |
| Transient expression vector | pCDNA-mNG-Talin-shRNA-R8 | R8 mutant of Talin1 resistant to shRNA. | ||
| Transient expression vector | pCDNA-mNG-shRNA-Talin | WT Talin1 resistant to shRNA | ||
| Transient expression vector | pCDNA-mNG-Talin | WT Talin1 | ||
| Lentiviral expression vector | pLVXCMV100-mNG-Talin-shRNA-R8 | R8 mutant of Talin1 resistant to shRNA. | ||
| Lentiviral expression vector | pLVXCMV100-mNG-Talin-shRNA | WT Talin1 resistant to shRNA | ||
| Lentiviral expression vector | pLVXCMV100-mNG-Talin | WT Talin1 | ||
| Transient expression vector | Paxillin-GFP | Original construct from Horwitz Lab | ||
| Transient expression vector | Vinculin-GFP | Original construct from Horwitz Lab | ||
| Transient expression vector | Talin-GFP | Original construct from Horwitz Lab | ||
| Recombinant expression vector | pet151-Talin1-R7R8-WT (murine) | GeneArt | Original construct from Goult Lab | |
| Recombinant expression vector | pet151-Talin1-R7R8-VVV (murine) | GeneArt | Original construct from Goult Lab | |
| Recombinant expression vector | pet151-Vinculin-Vd1 (murine) | Original construct from Goult Lab | ||
| Synthetic peptide | RIAM-TBS1 peptide (RIAM_6_30-C) DIDQMFSTLLGEMDLLTQSLGVDT-C |
GLBiochem | ||
| Synthetic peptide | DLC1 peptide (DLC1_465_489-C) IFPELDDILYHVKGMQRIVNQWSEK-C |
GLBiochem |